ControlledModulation of Folate Polyglutamyl Tail Length by Metabolic Engineeringof Lactococcuslactis
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چکیده
منابع مشابه
Controlled modulation of folate polyglutamyl tail length by metabolic engineering of Lactococcus lactis.
The dairy starter bacterium Lactococcus lactis is able to synthesize folate and accumulates >90% of the produced folate intracellularly, predominantly in the polyglutamyl form. Approximately 10% of the produced folate is released into the environment. Overexpression of folC in L. lactis led to an increase in the length of the polyglutamyl tail from the predominant 4, 5, and 6 glutamate residues...
متن کاملAbsorption and utilization of polyglutamyl forms of folate in man.
Some three-quarters of folate compounds in a normal mixed diet have a chain of seven glutamic acid residues (polyglutamates). The extent to which these forms of folate are absorbed and utilized by man is of considerable nutritional importance. These studies indicate that the polyglutamate forms were absorbed and utilized to about one-third of the extent of simpler (monoglutamate) forms, as judg...
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(Z)-Tetrahydropteroyltriglutamate and (Z)-tetrahydropteroylhexaglutamate were prepared and tested as substrates for thymidylate synthetase (EC 2.1.1.6) (methylenetetrahydrofolate: deoxyuridylate C-methyltransferase) from Zactobacillus casei. Both tetrahydropteroylpolyglutamates were more effective substrates than (Z)-tetrahydropteroylglutamate, enhancing the reaction rate 3-fold when compared a...
متن کاملIncreased production of folate by metabolic engineering of Lactococcus lactis.
The dairy starter bacterium Lactococcus lactis is able to synthesize folate and accumulates large amounts of folate, predominantly in the polyglutamyl form. Only small amounts of the produced folate are released in the extracellular medium. Five genes involved in folate biosynthesis were identified in a folate gene cluster in L. lactis MG1363: folA, folB, folKE, folP, and folC. The gene folKE e...
متن کاملFolate metabolic pathways in Leishmania.
Trypanosomatid parasitic protozoans of the genus Leishmania are autotrophic for both folate and unconjugated pteridines. Leishmania salvage these metabolites from their mammalian hosts and insect vectors through multiple transporters. Within the parasite, folates are reduced by a bifunctional DHFR (dihydrofolate reductase)-TS (thymidylate synthase) and by a novel PTR1 (pteridine reductase 1), w...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2003
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.69.12.7101-7107.2003